Author(s): Vishal Jain

Email(s): vishaljain123@gmail.com

DOI: 10.52711/2321-581X.2023.00005   

Address: Vishal Jain*
University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)
*Corresponding Author

Published In:   Volume - 14,      Issue - 2,     Year - 2023


ABSTRACT:
Bhuvnesvara vati is an important Ayurvedic formulation, is official in Ayurvedic formulary of India. The present study is an attempt to develop Fingerprint method for Bhuvnesvara vati with High Performance Liquid chromatography (HPLC) using gallic acid and tannic acid as a standard was developed, which are important and major content in formulation. The RP- HPLC Fingerprinting method was developed for three laboratory batch of Bhuvnesvara vati, its two marketed formulations and for its raw materials Emblica officinalis, Terminalia belerica, Terminalia chebula, Trichyspermum ammi and Aegle marmelus.The concentration of gallic acid present in raw material is found to be 3.174±0.049% w/w in Emblica officinalis, 8.920±0.173% w/w in Terminalia belerica, 4.092±0.117% w/w in Terminalia chebula, 1.831±0.973% w/w in Aegle marmelus and 0.264±0.365% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356% and 2.632±0.239% w/w respectively. Two marketed formulation of Bhuvnesvara vati M-I and M-II showed gallic acid concentration to be 2.019±0.872 % and 2.019±0.872 % w/w respectively. The concentration of tannic acid present in raw material was found to be 6.172%±0.365w/w in Emblica officinalis, 8.667%±0.0319w/w in Terminalia belerica, 13.956%±0.745w/w in Terminalia chebula, 4.789±0.983% w/w in Aegle marmelus and 0.668±1.002% w/w in Trichyspermum ammi respectively and in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356%, 2.632±0.239% w/w respectively. The HPLC method developed for the simultaneous estimation of gallic acid and tannic acid is a simple, rapid and precise for the routine estimation of Bhuvnesvara vati.


Cite this article:
Vishal Jain. Development of HPLC fingerprinting method via estimation of gallic acid and tannic acid for routine quality control of Ayurvedic formulation Bhuvnesvara vati. Research Journal of Engineering and Technology. 2023; 14(2):51-6. doi: 10.52711/2321-581X.2023.00005

Cite(Electronic):
Vishal Jain. Development of HPLC fingerprinting method via estimation of gallic acid and tannic acid for routine quality control of Ayurvedic formulation Bhuvnesvara vati. Research Journal of Engineering and Technology. 2023; 14(2):51-6. doi: 10.52711/2321-581X.2023.00005   Available on: https://www.ijersonline.org/AbstractView.aspx?PID=2023-14-2-1


REFERENCES:
1.    The Ayurvedic Formulary of India. Part II, 1st edition. Delhi: Govt. of India, Ministry of Health and Family Planning, Dept. of Health; 2000:178.
2.    Bhaisajyaratnavali. 2nd edition. Varanasi: Chowkhamba Sanskrit Series Office; 1961: Sloke no.149-150,163.
3.    Tripti Jain, and Kamlesh Dashora, Spectrophotometric fingerprinting method for Unani formulation Hab-e-Azarakhi. Asian J. Pharm. Tech. 2012; 2(1): 1-3
4.    Tripti Jain, Amber Vyas, Darshan Dubey, Kamlesh Dashora, Vishal Jain, Development of fingerprinting method for Siddha formulation Nilaavaarai chooranam: A HPTLC approach. Asian J. Research Chem. 2022; 15(5): 327-330.
5.    Vishal Jain, Ambar Vyas, Swarnlata Saraf, S. Saraf. HPLC Determination of Piperine in ‘Trikatu Churna’ a Potent Ayurvedic Formulation for Routine Quality Control. Asian J. Research Chem. 2011; 4(2): 183-186.  
6.    World Health Organization, Quality Control Methods For Medicinal Plants Materials, Geneva, 1998, 1-15.
7.    WHO General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine. (2000): http://whqlibdoc.who.int/hq/2000/WHO_EDM_TRM_2000.1.pdf.

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