ABSTRACT:
Bhuvnesvara vati is an important Ayurvedic formulation, is official in Ayurvedic formulary of India. The present study is an attempt to develop Fingerprint method for Bhuvnesvara vati with High Performance Liquid chromatography (HPLC) using gallic acid and tannic acid as a standard was developed, which are important and major content in formulation. The RP- HPLC Fingerprinting method was developed for three laboratory batch of Bhuvnesvara vati, its two marketed formulations and for its raw materials Emblica officinalis, Terminalia belerica, Terminalia chebula, Trichyspermum ammi and Aegle marmelus.The concentration of gallic acid present in raw material is found to be 3.174±0.049% w/w in Emblica officinalis, 8.920±0.173% w/w in Terminalia belerica, 4.092±0.117% w/w in Terminalia chebula, 1.831±0.973% w/w in Aegle marmelus and 0.264±0.365% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356% and 2.632±0.239% w/w respectively. Two marketed formulation of Bhuvnesvara vati M-I and M-II showed gallic acid concentration to be 2.019±0.872 % and 2.019±0.872 % w/w respectively. The concentration of tannic acid present in raw material was found to be 6.172%±0.365w/w in Emblica officinalis, 8.667%±0.0319w/w in Terminalia belerica, 13.956%±0.745w/w in Terminalia chebula, 4.789±0.983% w/w in Aegle marmelus and 0.668±1.002% w/w in Trichyspermum ammi respectively and in three identical laboratory batch of Bhuvnesvara vati BV-I, BV-II and BV-III, was found to be 2.623±0.746%, 2.589±0.356%, 2.632±0.239% w/w respectively. The HPLC method developed for the simultaneous estimation of gallic acid and tannic acid is a simple, rapid and precise for the routine estimation of Bhuvnesvara vati.
Cite this article:
Vishal Jain. Development of HPLC fingerprinting method via estimation of gallic acid and tannic acid for routine quality control of Ayurvedic formulation Bhuvnesvara vati. Research Journal of Engineering and Technology. 2023; 14(2):51-6. doi: 10.52711/2321-581X.2023.00005
Cite(Electronic):
Vishal Jain. Development of HPLC fingerprinting method via estimation of gallic acid and tannic acid for routine quality control of Ayurvedic formulation Bhuvnesvara vati. Research Journal of Engineering and Technology. 2023; 14(2):51-6. doi: 10.52711/2321-581X.2023.00005 Available on: https://www.ijersonline.org/AbstractView.aspx?PID=2023-14-2-1